BioMed UQAM
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Le Dr Nicolas Pilon est titulaire d’un B.Sc. en biochimie de l’Université de Sherbrooke (1995) et d’un Ph.D. en biologie moléculaire de l’Université de Montréal (2001). Afin de poursuivre sa formation de chercheur en biologie du développement, il a effectué un premier stage post-doctoral à l’Institut de recherches cliniques de Montréal où il s’est intéressé à la régulation et la fonction des facteurs de transcription Cdx dans le processus de régionalisation de l’axe antérieur-postérieur chez la souris. Au cours d’un deuxième stage post-doctoral à l’Université de Montréal, il s’est intéressé à la caractérisation de mutants génétiques ayant des malformations congénitales résultant de défauts au niveau des cellules de la crête neurale. Le Dr Pilon s’est joint au corps (...) >  suite


Chez les vertébrés, une première ébauche du système nerveux central est obtenue par la formation du tube neural (neurulation). Le tube neural est formé par l’élévation de plis neuraux latéraux à partir de la plaque neurale (neuro-ectoderme) suivi par le repliement et la fusion de ceux-ci. Comme l’indique la compartimentalisation du tube neural en futur cerveau et moelle épinière, la neurulation est très finement couplée à des mécanismes de spécification de l’identité positionnelle des cellules le long de l’axe antérieur-postérieure. De plus, la neurulation est coordonnée avec l’induction d’une population particulière de cellules aux extrémités des plis neuraux : les cellules de la crête neurale (CCN). Suite à la fermeture du tube neural, les CCN émigrent de sa (...) >  suite


Publications récentes


Induction and dorsal restriction of Paired-box 3 (Pax3) gene expression in the caudal neuroectoderm is mediated by integration of multiple pathways on a short neural crest enhancer

Sanchez-Ferras O, Bernas G, Laberge-Perrault E, Pilon N.

Biochim Biophys Acta - Gene Regulatory Mechanisms. 2014 May 9. [Epub ahead of print]

(21 mai 2014)
Pax3 encodes a paired-box transcription factor with key roles in neural crest and neural tube ontogenesis. Robust control of Pax3 neural expression is ensured by two redundant sets of cis-regulatory modules (CRMs) that integrate (...) suite

Characterization of a novel transgenic mouse line expressing Cre recombinase under the control of the Cdx2 neural specific enhancer.

Coutaud B, Pilon N.

Genesis. 2013 Aug 2:1-8. doi : 10.1002/dvg.22421. [Epub ahead of print]

(16 septembre 2013)
Several genetically modified mouse models have been generated in order to drive expression of the Cre recombinase in the neuroectoderm. However, none of them specifically targets the posterior neural plate during neurulation. To fill this gap, (...) suite

The developmental genetics of Hirschsprung’s disease

Bergeron KF, Silversides DW, Pilon N.

Clin Genet. 2012 Oct 8. doi : 10.1111/cge.12032. [Epub ahead of print]

(24 octobre 2012)
Hirschsprung’s disease, also known as aganglionic megacolon, derives from a congenital malformation of the enteric nervous system (ENS). It displays an incidence of 1 in 5000 live births with a 4:1 male to female sex ratio. Clinical signs include (...) suite

Caudal-related homeobox (Cdx) protein-dependent integration of canonical Wnt signaling on paired-box 3 (Pax3) neural crest enhancer.

Sanchez-Ferras O, Coutaud B, Djavanbakht Samani T, Tremblay I, Souchkova O, Pilon N.

J Biol Chem. 2012 May 11 ;287(20):16623-35. Epub 2012 Mar 28.

(24 octobre 2012)
One of the earliest events in neural crest development takes place at the neural plate border and consists in the induction of Pax3 expression by posteriorizing Wnt·β-catenin signaling. The molecular mechanism of this regulation is not well (...) suite

Transgenic mouse analysis of Sry expression during the pre- and peri-implantation stage

Silversides DW, Raiwet DL, Souchkova O, Viger RS, Pilon N.

Dev Dyn. 2012 Jul ;241(7):1192-204. doi : 10.1002/dvdy.23798. Epub 2012 May 18.

(24 octobre 2012)
BACKGROUND : The SRY/Sry gene is expressed in pre-Sertoli cells of the male genital ridge and functions as the mammalian testis determining factor (TDF). In addition, expression of SRY/Sry outside the genital ridge has been reported, including (...) suite

An Ebox element in the proximal Gata4 promoter is required for Gata4 expression in vivo

Boulende Sab A, Bouchard MF, Béland M, Prud’homme B, Souchkova O, Viger RS, Pilon N.

PLoS One. 2011 ;6(12):e29038. Epub 2011 Dec 13.

(24 octobre 2012)
GATA4 is an essential transcription factor required for the development and function of multiple tissues, including a major role in gonadogenesis. Despite its crucial role, the molecular mechanisms that regulate Gata4 expression in vivo remain (...) suite

Cdx1 and Cdx2 are functionally equivalent in vertebral patterning
Savory JG*, Pilon N*, Sylvestre JR, Béland M, Houle M and D Lohnes (*equivalent contribution) (27 mars 2009)
The Cdx transcription factors regulate anterior-posterior (AP) vertebral patterning, at least in part, through direct regulation of Hox gene expression. Analysis of allelic series of Cdx mutant mice suggests functional overlap between these (...) suite

Novel pre- and post-gastrulation expression of Gata4 within cells of the inner cell mass and migratory neural crest cells.
Pilon N, Raiwet D, Viger RS, Silversides DW. (19 mars 2008)
GATA4 is a transcription factor known to be important for the development of many organs such as the heart, intestine, and gonads. However, information regarding the control of its expression is only now beginning to emerge. To further (...) suite

Wnt signaling is a key mediator of Cdx1 expression in vivo.
Pilon N, Oh K, Sylvestre JR, Savory JG, Lohnes D. (30 mai 2007)
Wnt signaling is a key mediator of Cdx1 expression in vivo. In the mouse, Cdx1 is essential for normal anteroposterior vertebral patterning through regulation of a subset of Hox genes. Retinoic acid (RA) and certain Wnts have also been (...) suite

Presumptive pre-Sertoli cells express genes involved in cell proliferation and cell signalling during a critical window in early testis differentiation.
Cory AT, Boyer A, Pilon N, Lussier JG, Silversides DW. (4 avril 2007)
In mammals, the pre-Sertoli cell of the male genital ridge is the first cell type to display sex specific differentiation and differential gene expression. The genetic cascade driving the differentiation of pre-Sertoli cells and ultimately (...) suite

The proximal Gata4 promoter directs reporter gene expression to sertoli cells during mouse gonadal development.
Mazaud Guittot S, Tétu A, Legault E, Pilon N, Silversides DW, Viger RS. (4 octobre 2006)
The GATA4 transcription factor is an important developmental determinant for many organs, such as the heart, gut, and testis. Despite this pivotal role, our understanding of the transcriptional mechanisms that control the proper spatiotemporal (...) suite

Human and pig SRY 5 flanking sequences can direct reporter transgene expression to the genital ridge and to migrating neural crest cells
Boyer A, Pilon N, Raiwet DL, Lussier JG, Silversides DW. (12 janvier 2006)
Human and pig SRY 5 flanking sequences can direct reporter transgene expression to the genital ridge and to migrating neural crest cells Mechanisms for sex determination vary greatly between animal groups, and include chromosome dosage and (...) suite

Cdx4 is a direct target for the canonical Wnt signaling pathway
Pilon N, Oh K, Sylvestre JR, Bouchard N, Savory J, Lohnes D. (1er janvier 2006)
Cdx4 is a direct target for the canonical Wnt signaling pathway There is considerable evidence that the Cdx gene products impact on vertebral patterning by direct regulation of Hox gene expression. Data from a number of vertebrate model (...) suite

Cdx1 autoregulation is governed by a novel Cdx1-LEF1 transcription complex.
Béland M, Pilon N, Houle M, Oh K, Sylvestre JR, Prinos P, Lohnes D. †M.B. and N.P. contributed equally to this work. (24 juin 2004)
Cdx1 autoregulation is governed by a novel Cdx1-LEF1 transcription complex. The Cdx1 gene product is essential for normal anterior-posterior vertebral patterning. Expression of Cdx1 is regulated by several pathways implicated in (...) suite

Porcine SRY promoter is a target for steroidogenic factor 1.
Pilon N, Daneau I, Paradis V, Hamel F, Lussier JG, Viger RS, Silversides DW. (31 octobre 2002)
To study the process of mammalian sex determination and in particular to further understand the mechanisms of transcriptional regulation of the SRY gene, we have isolated a 4.5-kilobase (kb) pig SRY 5’ flanking sequence. To facilitate the in (...) suite

The porcine SRY promoter is transactivated within a male genital ridge environment.
Daneau I, Pilon N, Boyer A, Behdjani R, Overbeek PA, Viger R, Lussier J, Silversides DW. (6 août 2002)
In mammals the SRY gene functions as a dominant genetic switch for testis determination (Gubbay et al. : Nature 346:1128-1135, 1990 ; Koopman et al. : Nature 351:117-121, 1991 ; Sinclair et al. : Nature 346:240-244, 1990). To study SRY (...) suite

Genetic manipulation of sex differentiation and phenotype in domestic animals.
Silversides DW, Pilon N, Behdjani R, Boyer A, Daneau I, Lussier J. (26 mars 2001)
In mammals, a gene based sex determination system ensures that approximately 50% of offspring will be of the male sex and 50% will be of the female sex. In domestic animal production systems, this ratio is not always ideal. Recent advances in (...) suite

Expression and regulation of transcripts encoding two members of the NR5A nuclear receptor subfamily of orphan nuclear receptors, steroidogenic factor-1 and NR5A2, in equine ovarian cells during the ovulatory process.
Boerboom D, Pilon N, Behdjani R, Silversides DW, Sirois J. (décembre 2000)
Steroidogenic factor-1 (SF-1, NR5A1a) is a member of the NR5A nuclear receptor subfamily and has been implicated as a key transcriptional regulator of all ovarian steroidogenic genes in vitro. To establish links between the expression of SF-1 (...) suite

Analysis of T cell receptor beta chain expression by isoelectric focusing following gene amplification and in vitro translation.
Bouffard P, Gagnon C, Cloutier D, MacLean SJ, Souleimani A, Nallainathan D, Home WA, Pilon N, Gibson DM. (janvier 2000)
We describe a new approach to analysis of T cell receptor diversity based on isoelectric focusing of in vitro translation products of amplified V region genes. The method is illustrated by analysis of V beta 2 profiles in peripheral blood (...) suite

Porcine steroidogenic factor-1 gene (pSF-1) expression and analysis of embryonic pig gonads during sexual differentiation.
Pilon N, Behdjani R, Daneau I, Lussier JG, Silversides DW. (septembre 1998)
The porcine steroidogenic factor-1 gene (pSF-1) was cloned using a combination of genomic and RT-PCR based cloning methods. pSF-1 consists of an open reading frame of 1383 nt corresponding to a deduced amino acid sequence of 461 aa, similar to (...) suite

Porcine and bovine steroidogenic acute regulatory protein (StAR) gene expression during gestation.
Pilon N, Daneau I, Brisson C, Ethier JF, Lussier JG, Silversides DW. (mars 1997)
We have generated complete complementary DNA (cDNA) sequences for the porcine steroidogenic acute regulatory protein (StAR) gene, using a combination of genomic PCR amplification and reverse transcription-PCR amplification of pig ovarian cDNA. (...) suite


Équipe

Membres actuels :

  • Ouliana SOUCHKOVA, agente de recherche
  • Guillaume BERNAS, agent de recherche
  • Dr Karl F. BERGERON, stagiaire postdoctoral
  • Dr Rodolphe SORET, stagiaire postdoctoral
  • Dr Omar FARNOS-VILLAR, stagiaire postdoctoral
  • Tatiana CARDINAL, étudiante PhD Biochimie UQAM, Boursière IRSC et Fondation GO
  • Oraly SANCHEZ-FERRAS, étudiante PhD Biochimie UQAM, Boursière CRSNG AG Bell
  • Aboubacrine M. TOURÉ, étudiant PhD Biochimie UQAM, Boursier FRQNT-étudiants étrangers
  • Alain BOULENDE, étudiant PhD Biologie UQAM
  • Daniel Efrain AGUIRRE, étudiant MSc Biochimie UQAM
  • Emilie LABERGE-PERRAULT, étudiante MSc Biologie moléculaire UdeM
  • Chloé NGUYEN, étudiante MSc Sciences Biomédicales UdeM
  • Catherine BÉLANGER, étudiante MSc Biochimie UQAM

Stagiaires 1er cycle :

  • Adrien PERALDO, Université Paris 7 Denis Diderot, 2014
  • Mathilde MENNETREY, IUT de Dijon (France), 2014
  • Renaud DION, BSc Biochimie, UQAM, 2014
  • Vanessa DESCHAMBAULT, BSc Biologie, UQAM, 2014
  • Baptiste CHARRIER, BSc Biologie, UQAM, 2014
  • Catherine BÉLANGER, BSc Biologie UQAM, 2014
  • Marina CÉFIS, IUT de Dijon (France), 2013
  • Chloé NGUYEN, BSc Biologie, UQAM, 2013
  • Emilie LABERGE-PERRAULT, BSc Biologie, UQAM, 2013
  • Victoria LECORVEC, DUT Génie Biologique, IUT de Brest (France), 2012
  • Isabelle TREMBLAY, BSc Biochimie, UQAM, Bourses CRSNG BRPC 2010 et 2011
  • Tatiana CARDINAL, BSc Biotechnologie, La cité collégiale d’Ottawa, 2011
  • Luciano OTERO, BSc Biochimie, UQAM, 2011
  • Marine BERSOULT, DUT Génie Biologique, Université Claude-Bernard (Lyon, France), 2011

Anciens membres :

  • Baptiste COUTAUD, MSc Biologie UQAM (2010-2013)
  • Mélanie BÉLAND, chercheure associée (2009-2012)
  • Taraneh D. SAMANI, MSc Biologie UQAM (2008-2010)
  • Hiroaki TANIGUCHI, stagiaire postdoctoral (2009)

Contacts

Nicolas PILON, Ph.D.

  • Professeur
  • Dépt. des Sciences Biologiques,
    Université du Québec à Montréal,
    C.P. 8888, Succursale Centre-Ville, Montréal, Canada, H3C 3P8
  • Bureau : SB-3365
  • Locaux de recherche : SB-3350, SB-3360 et SB-3430
  • Tél. bureau : (514) 987-3000, poste 3342
  • Tél. labo SB-3360 : (514) 987-3000, poste 7922
  • Tél. labo SB-3430 : (514) 987-3000, poste 4755
  • Télécopieur : (514) 987-4647
  • Courriel
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